Home / Integrin alpha 1/CD49a / Human Integrin  alpha 1/CD49a Antibody

Human Integrin  alpha 1/CD49a Antibody

Catalog #: MAB5676
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5676
MAB5676-SP
Detection of Human Integrin alpha 1/CD49a by Western Blot.
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha 1/CD49a Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/ beta 4, alpha D/ beta 2, alpha 7x2/ beta 1, alpha E/ beta 7, or alpha 2b/ beta 3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 639508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin a1/CD49a antibody by Western Blot. View Larger

Detection of Human Integrin alpha 1/CD49a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin a1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Integrin alpha 1/CD49a by Western Blot View Larger

Detection of Human Integrin alpha 1/CD49a by Western Blot Levels of HAV RNA at 24 hpi in H1-HeLa cells engineered by CRISPR/Cas9 to be knocked-out for the indicated integrins inoculated with naked HAV (mean ± SD).Immunoblots of whole-cell lysates harvested from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30801249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1. 

Entrez Gene IDs
3672 (Human); 109700 (Mouse); 25118 (Rat)
Alternate Names
CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1

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Citations for Human Integrin  alpha 1/CD49a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer
    Authors: Hongfei Yan, Chunlei Zheng, Zhi Li, Bowen Bao, Bowen Yang, Kezuo Hou et al.
    Cancer Management and Research
  2. FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments
    Authors: LB Gong, C Zhang, RX Yu, C Li, YB Fan, YP Liu, XJ Qu
    Onco Targets Ther, 2020-07-28;13(0):7399-7409.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. α11?1 Integrin is Induced in a Subset of Cancer-Associated Fibroblasts in Desmoplastic Tumor Stroma and Mediates In Vitro Cell Migration
    Authors: C Zeltz, J Alam, H Liu, PM Erusappan, H Hoschuetzk, A Molven, H Parajuli, E Cukierman, DE Costea, N Lu, D Gullberg
    Cancers (Basel), 2019-06-01;11(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3
    Authors: WS Chen, Z Cao, S Sugaya, MJ Lopez, VG Sendra, N Laver, H Leffler, UJ Nilsson, J Fu, J Song, L Xia, P Hamrah, N Panjwani
    Nat Commun, 2016-04-12;7(0):11302.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses
    Authors: Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM.
    Elife
  6. Collagen-rich omentum is a premetastatic niche for integrin alpha 2-mediated peritoneal metastasis
    Authors: Yen-Lin Huang, Ching-Yeu Liang, Danilo Ritz, Ricardo Coelho, Dedy Septiadi, Manuela Estermann et al.
    eLife

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Human Integrin  alpha 1/CD49a Antibody
By Anonymous on 04/30/2018
Application: WB Sample Tested: Huh-7 human hepatoma cell line Species: Human