Human Integrin alpha 4/CD49d Antibody

Catalog #: MAB13541 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13541-100
MAB13541-SP

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Human Integrin alpha 4/CD49d Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 4/CD49d in direct ELISA. In a sandwich immunoassay, this antibody detects the human Integrin alpha 4 beta 1 heterodimer when used with the suggested detection antibody, Mouse Anti-Human Integrin beta 1 (Catalog # MAB17784).

Source

Monoclonal Mouse IgG₁ Clone # 599904

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 4/CD49d
Tyr34-Gln970 (Arg591Leu & Arg878Gln)
Accession # P13612

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody the human Integrin alpha 4 beta 1 heterodimer when paired with Mouse Anti-Human Integrin beta 1 Monoclonal Antibody (Catalog # MAB17784).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4