Home / Integrin alpha V/CD51 / Human Integrin  alpha V/CD51 Antibody

Human Integrin  alpha V/CD51 Antibody

Catalog #: MAB1219
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1219
MAB1219-SP
Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha V/CD51 Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of human Integrin alpha V/CD51.
Source
Monoclonal Mouse IgG1 Clone # P2W7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Ocular melanoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin aV/CD51 antibody in HT1080 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).

Immunocytochemistry Integrin aV/CD51 antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha V/CD51 in HT1080 Human Cell Line. Integrin aV/CD51 was detected in immersion fixed HT1080 human fibrosarcoma cell line using 10 µg/mL Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alpha V (CD51), also known as Vitronectin Receptor Subunit alpha is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta ‑propellor domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

Entrez Gene IDs
3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

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Citations for Human Integrin  alpha V/CD51 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Interferon-Induced Transmembrane Proteins Inhibit Infection by the Kaposi's Sarcoma-Associated Herpesvirus and the Related Rhesus Monkey Rhadinovirus in a Cell-Specific Manner
    Authors: BF Hörnich, AK Gro beta kopf, CJ Dcosta, S Schlagowsk, AS Hahn
    MBio, 2021-12-21;12(6):e0211321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Plasma endothelial microparticles reflect the extent of capillaroscopic alterations and correlate with the severity of skin involvement in systemic sclerosis
    Authors: Dorota Krasowska
    Microvasc. Res., 2016-11-23;110(0):24-31.
    Species: Human
    Sample Types: Platelet Poor Plasma
    Applications: Flow Cytometry
  3. Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via ?v?3 Integrin
    Authors: S Cornick, F Moreau, K Chadee
    PLoS Pathog, 2016-04-13;12(4):e1005579.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Detection)
  4. Heparin inhibits the interaction of DNA topoisomerase I/anti-topoisomerase I immune complexes with heparan sulfate on dermal fibroblasts.
    Authors: Arcand J, Robitaille G, Koenig M, Senecal J, Raymond Y
    Arthritis Rheum, 2012-05-01;64(5):1632-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
    Authors: Tatrai P, Dudas J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
    Lab. Invest., 2006-09-18;86(11):1149-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. BMP2 is Superior to BMP4 for Promoting Human Muscle-Derived Stem Cell-Mediated Bone Regeneration in a Critical-Sized Calvarial Defect Model
    Authors: Xueqin Gao, Arvydas Usas, Aiping Lu, Ying Tang, Bing Wang, Chien-Wen Chen et al.
    Cell Transplantation

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Human Integrin  alpha V/CD51 Antibody
By Anonymous on 04/06/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HT1080 human fibrosarcoma cell line Species: Human
Immunocytochemistry/Immunofluorescence Human Integrin  alpha V/CD51 Antibody MAB1219