Human Integrin beta 1/CD29 Biotinylated Antibody

Name: Human Integrin beta 1/CD29 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1778
Price: 569 USD
Availability: InStock

R&D Systems | Catalog # BAF1778

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Citations (9)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot, Bioassay, ELISA Detection, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556

Specificity

Detects human Integrin beta 1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Integrin beta 1 is observed and less than 1% cross-reactivity recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, rmIntegrin beta 6, and rmIntegrin beta 7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin beta 1/CD29 Biotinylated Antibody

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human Integrin beta 1/CD29 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1778, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Applications for Human Integrin beta 1/CD29 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin beta 1/CD29

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes.