Human Integrin beta 5 Antibody Summary
Gly24-Asn719
Accession # P18084
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Integrin beta 5 in MG‑63 Human Cell Line. Integrin beta 5 was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Integrin beta 5 by Simple WesternTM. Simple Western lane view shows lysates of MG‑63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 5 at approximately 129 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human Integrin beta 5 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin beta 5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 5
Integrin beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alpha V. Integrin alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta 5, respectively.
Product Datasheets
Citations for Human Integrin beta 5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model
Authors: Hong Yien Tan, Yean Kong Yong, Yuan Chao Xue, Huitao Liu, Tomomi Furihata, Esaki Muthu Shankar et al.
iScience
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Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer.
Authors: Lin Z, Zhang C, Zhang M, Xu D, Fang Y, Zhou Z, Chen X, Qin N, Zhang X
PLoS ONE, 2014-01-20;9(1):e85296.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Purification of rat and mouse astrocytes by immunopanning
Authors: Lynette C Foo
Cold Spring Harb Protoc
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Development of a method for the purification and culture of rodent astrocytes.
Authors: Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA
Neuron, 2011-09-08;71(5):799-811.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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MDA-MB-231 cells were growm on collagen coated coverslips for 12 hours.
