Human Integrin beta 7 Fluorescein-conjugated Antibody

Catalog #: FAB4669F
1 Citation Datasheet / COA / SDS

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FAB4669F

Detection of Integrin beta 7 in PBMCs Lymphocytes by Flow Cytometry.

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Human Integrin beta 7 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 8, recombinant mouse Integrin beta 4, or beta 7 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 473207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
Glu20-His723
Accession # P26010

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 7 in PBMCs Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta 7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4669F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 7

Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20‑723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150‑389) and four cysteine‑rich EGF‑like repeats. One splice variant exists that shows a deletion of aa 501‑648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1 and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.

Entrez Gene IDs

3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)

Alternate Names

Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7

Product Datasheets

Product Datasheet

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Citation for Human Integrin beta 7 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration.
Authors: Sereti I, Estes J, Thompson W, Morcock D, Fischl M, Croughs T, Beq S, Lafaye de Micheaux S, Yao M, Ober A, Wilson E, Natarajan V, Imamichi H, Boulassel M, Lederman M, Routy J
PLoS Pathog, 2014-01-30;10(1):e1003890.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry