Human IRF2 Antibody

Catalog #: AF4049 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4049
AF4049-SP

Detection of Human IRF2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IRF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IRF2
Val151-Cys349
Accession # P14316

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IRF2 by Western Blot. Western blot shows nuclear extracts of LNCaP human prostate cancer cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human IRF2 by Simple Western^TM. Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IRF2 at approximately 61 kDa (as indicated) using 5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF2

Interferon Regulatory Factor 2 (IRF2) belong to the interferon regulatory transcription factor family. IRF2 functions as a regulator of type I interferons influencing cellular proliferation and immune response through transcriptional regulation. IRF2 competitively inhibits IRF1 gene activation, as well as, stimulating transcription on its own. Over-expression of IRF2 in NIH3T3 cells leads to transformation, whereas, loss of IRF2 expression in knock out mice leads to a variety of abnormalities including loss of NK cells due to increased apoptosis.

Long Name

Interferon Regulatory Factor 2

Entrez Gene IDs

3660 (Human); 16363 (Mouse); 290749 (Rat)

Alternate Names

DKFZp686F0244; interferon regulatory factor 2; IRF2; IRF-2