Human Islet-2 Biotinylated Antibody

Catalog #: BAF4244 Datasheet / COA / SDS

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BAF4244

All Islet-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Islet-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Islet-2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Islet-2
Met1-Thr359
Accession # Q96A47

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Islet‑2

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of embyronic mouse neural tube (E13.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Islet-2

Islet-2 is a member of the LIM homeodomain transcription factors and its expression pattern defines a subclass of motor neurons. Islet-2 contains two amino terminal LIM domains and a carboxy terminal homeodomain. Human Islet-2 shares 93% similarity with mouse Islet-2.

Entrez Gene IDs

64843 (Human); 104360 (Mouse); 57233 (Rat)

Alternate Names

FLJ10160; insulin gene enhancer protein ISL-2; ISL LIM homeobox 2; ISL2 transcription factor, LIM/homeodomain, (islet-2); ISL2; Islet2; Islet-2