Human ITM2A Antibody

Catalog #: AF4876 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4876
AF4876-SP

Detection of Human ITM2A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITM2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITM2A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ITM2B is observed, and less than 1% cross-reactivity with rhITM2C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ITM2A
Pro80-Glu263
Accession # O43736

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Human cartilage tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ITM2A by Western Blot. Western Blot shows lysates of A‑673 human Ewing's sarcoma cell line. PVDF membrane was probed with 2 µg/ml of Sheep Anti-Human ITM2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4876) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2A at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITM2A

ITM2A (Integral membrane protein 2A; also protein E25) is a 43-45 kDa member of the ITM2 family of proteins. It is expressed by chondrocytes involved in endochondrial ossification, skeletal muscle, adipose tissue-derived stem cells, and CD3-activated CD4⁺ and CD8⁺ T cells. ITM2A is found embedded in both plasma membrane and Golgi complex. Human ITM2A is a 263 amino acid (aa) type II transmembrane glycoprotein. The ECD (aa 75-263) contains one BRICHOS domain (aa 133‑227). Proteolytic cleavage by furin is typical for BRICHOS domain-containing proteins. ITM2A may exist as a dimer. There are two potential splice variants, one that shows a deletion of aa 55-81, and a second that shows a deletion of aa 38-81. Full-length human ITM2A shares 95% aa identity with mouse ITM2A.

Long Name

Integral Membrane Protein 2A

Entrez Gene IDs

9452 (Human); 16431 (Mouse); 317218 (Rat)

Alternate Names

BRICD2A; BRICHOS domain containing 2A; E25A; E25ABRICD2A; integral membrane protein 2A; ITM2A; Protein E25