Human JAB1 Antibody

Catalog #: MAB6390 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6390-100
MAB6390-SP

Detection of Human JAB1 by Western Blot.

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Human JAB1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human JAB1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 687409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human JAB1
Asn158-Ser334
Accession # Q92905

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human JAB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human JAB1 Monoclonal Antibody (Catalog # MAB6390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for JAB1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAB1

JAB1 (Jun activation domain binding protein-1), also called COPS5 or CSN5 (COP9 signalosome-5), is a 334 amino acid (aa), 38 kDa protein that is a subunit of the COP9 signalosome complex. It mediates cytosolic translocation of the cell cycle inhibitory protein, CDKN1B/p27Kip1, for degradation, thus promoting proliferation. It is widely expressed, especially in proliferating cells and up-regulates granulopoiesis when overexpressed. Within the region used as an immunogen, human JAB1 shares 99% aa identity with mouse and rat JAB1. A 379 aa isoform contains 93 alternate aa in place of the N-terminal 48 aa of the 334 aa form.

Long Name

Jun Activation Domain-binding Protein 1

Entrez Gene IDs

10987 (Human); 26754 (Mouse); 312916 (Rat)

Alternate Names

COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis); COP9 signalosome complex subunit 5; COPS5; CSN5; CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5; EC 3.4; JAB1; JAB138 kDa Mov34 homolog; Jun activation domain-binding protein 1; MOV-34; SGN5; SGN5MGC3149; Signalosome subunit 5