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Human JAM-A Biotinylated Antibody

Catalog #: BAF1103
2 Citations Datasheet / COA / SDS
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BAF1103
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Citations (2)
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Human JAM-A Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human JAM-A in Western blots. In this format, approximately 15% cross-reactivity with recombinant mouse JAM-A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-A
Ser28-Ala242
Accession # Q9Y624
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human JAM-A Fc Chimera (Catalog # 1103-JM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1‑4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig‑like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM‑A‑LFA‑1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Sobocka, M.B. et al. (2000) Blood 95:2600.
  4. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  5. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
  6. Ostermann, G. et al. (2002) Nature Immunol. 3:151.
  7. Barton, E.S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Entrez Gene IDs
50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

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Citations for Human JAM-A Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
    Authors: Williams D, Anastos K, Morgello S, Berman J
    J Leukoc Biol, 2014-11-24;97(2):401-12.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  2. Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
    PLoS ONE, 2010-10-21;5(10):e13568.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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