Home / Kallikrein 5 / Human Kallikrein 5 Antibody

Human Kallikrein 5 Antibody

Catalog #: AF1108
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1108
AF1108-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse KLK5 is observed and less than 10% cross-reactivity with recombinant human KLK8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5
Ile67-Ser293
Accession # Q9Y337
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kallikrein 5 (Catalog # 1108-SE)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 5 (Catalog # 1108-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

References
  1. Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
  2. Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
  3. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  4. Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
  5. Kim, H. et al. (2001) Br. J. Cancer 84:643.
Entrez Gene IDs
25818 (Human); 68668 (Mouse); 690603 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme

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Citations for Human Kallikrein 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Kallikreins: Essential epidermal messengers for regulation of the skin microenvironment during homeostasis, repair and disease
    Authors: Pauline Nauroy, Alexander Nyström
    Matrix Biology Plus
  2. Cross-linking of SPINK6 by transglutaminases protects from epidermal proteases.
    Authors: Fischer J, Koblyakova Y, Latendorf T, Wu Z, Meyer-Hoffert U
    J Invest Dermatol, 2013-01-10;133(5):1170-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. alpha 2 Integrin-Dependent Suppression of Pancreatic Adenocarcinoma Cell Invasion Involves Ectodomain Regulation of Kallikrein-Related Peptidase-5
    Authors: Chia-Yao Lee, David Marzan, Grace Lin, Steve Goodison, Steve Silletti
    Journal of Oncology
  4. Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
    Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
    Biol. Chem., 2007-11-01;388(11):1235-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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