Human Kallikrein 5 Propeptide Antibody

Catalog #: MAB11082 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11082
MAB11082-SP

All Kallikrein 5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 5 Propeptide Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 5 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 5 (mature), rhKallikrein 1, -3, -4, -6, -7, -8, -9, -10, -11, -12, -13, -14, -15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin, or rhuPA-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 325302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5
Val23-Arg66
Accession # NP_036559

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kallikrein 5 (Catalog # 1108-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

References

Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
Kim, H. et al. (2001) Br. J. Cancer 84:643.

Entrez Gene IDs

25818 (Human); 68668 (Mouse); 690603 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme