Human KAP1 Antibody

Detection of Human and Mouse KAP1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for KAP1 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human KAP1 by Simple Western^TM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for KAP1 at approximately 117 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

KAP1 in Neuro‑2A Mouse Cell Line. KAP1 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

KAP1 in Human Liver Cancer Tissue. KAP1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of KAP1 in PC‑3 human prostate cancer cells KAP1 was detected in immersion fixed PC‑3 human prostate cancer cells using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.