Human Kell Antibody

Catalog #: AF1914 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1914
AF1914-SP

All Kell Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kell Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kell in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kell is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kell
aa 68-732

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Kell

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kell, see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kell

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

References

Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
Turner, A.J. et al. (2001) BioEssays. 23:261.
Lee, S. et al. (1999) Blood 94:1440.
Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Long Name

Zinc protease blood group antigen

Entrez Gene IDs

3792 (Human); 23925 (Mouse)

Alternate Names

CD238 antigen; CD238; EC 3.4.24.-; ECE3; KEL; kell blood group antigen; kell blood group glycoprotein; Kell blood group; Kell blood group, metalloendopeptidase; Kell blood group, metallo-endopeptidase; Kell