Human Kininogen Heavy Chain Antibody

Catalog #: MAB15692
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15692
MAB15692-SP

All Kininogen Antibodies

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Citations (1)

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Human Kininogen Heavy Chain Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kininogen Heavy Chain in direct ELISAs and Western blots. It reacts with HK, Kininogen, and the heavy chain, but not with the light chain alone. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E, S, SA, SN, rhFetuin A, rhFetuin B, rhHPRG, recombinant mouse Kininogen, or rhKininostatin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 236012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kininogen
Gln19-Ser644 (Ile581Thr)
Accession # P01042

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kininogen High Molecular Weight (HKa) (Catalog # 1569-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kininogen High Molecular Weight (HKa) (Catalog # 1569-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kininogen

Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18). The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains. The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases. The light chain consists of a His-rich domain, which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427). The LMW form is not involved in blood clotting.

References

Takagaki, Y. et al. (1985) J. Biol. Chem. 260:8601.

Entrez Gene IDs

3827 (Human); 16644 (Mouse); 24903 (Rat)

Alternate Names

alpha 2-thiol Proteinase Inhibitor; BDK; BDK;BK;HAE6;HK;HMWK;Kininogen-1;KNG;KNG1; Kininogen; KNG1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Kininogen Heavy Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1