Human KIR3DL2/CD158k Antibody

Catalog #: MAB2878
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2878
MAB2878-SP

Detection of KIR3DL2/CD158k in Human Blood Lymphocytes by Flow Cytometry.

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Citations (3)

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Human KIR3DL2/CD158k Antibody Summary

Species Reactivity

Human

Specificity

Detects human KIR3DL2/CD158k in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 539304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR3DL2/CD158k
Accession # P43630

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KIR3DL2/CD158k in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human KIR3DL2/CD158k Monoclonal Antibody (Catalog # MAB2878) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR3DL2/CD158k

KIR3DL2 (3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56^dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1-4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tails. Like other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid polymorphisms found in a single individual (4-6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8). It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2‑microglobulin (9, 10). NK and CD4⁺ T cells from patients with spondylarthritides show increased KIR3DL2⁺ expression and may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88-92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

References

Colonna, M. and J. Samaridis (1995) Science 268:405.
Pende, D. et al. (1996) J. Exp. Med. 184:505.
Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
Uhrberg, M. et al. (1997) Immunity 7:753.
Meenaugh, A. et al. (2004) Tiss. Antigens 64:226.
Kwon, D. et al. (2000) Mol. Cells 10:54.
Dohring, C. et al. (1996) J. Immunol. 156:3098.
Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2

Entrez Gene IDs

3812 (Human); 245615 (Mouse)

Alternate Names

killer cell immunoglobulin-like receptor 3DL2; CD158k; CL-5; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2; KIR3DL2; NKAT4; NKAT-4; NKAT4A; NKAT4B; p140

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Citations for Human KIR3DL2/CD158k Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Human NK Cells Downregulate Zap70 and Syk in Response to Prolonged Activation or DNA Damage
Authors: JL Pugh, N Nemat-Gorg, PJ Norman, LA Guethlein, P Parham
J. Immunol., 2017-12-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Depletion, Flow Cytometry
?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells
Authors: XH Zhang, XD Lian, ZX Dai, HY Zheng, X Chen, YT Zheng
J. Immunol., 2017-08-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The Incubation Period of Primary Epstein-Barr Virus Infection: Viral Dynamics and Immunologic Events.
Authors: Dunmire S, Grimm J, Schmeling D, Balfour H, Hogquist K
PLoS Pathog, 2015-12-01;11(12):e1005286.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry