Human KIR3DL2/CD158k PE-conjugated Antibody

Catalog #: FAB2878P
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2878P-025
FAB2878P-100

Detection of KIR3DL2/CD158k in Human PBMCs by Flow Cytometry.

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Human KIR3DL2/CD158k PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human KIR3DL2/CD158k in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 539304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human KIR3DL2/CD158k
Accession # P43630

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KIR3DL2/CD158k in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR3DL2/CD158k PE-conjugated Monoclonal Antibody (Catalog # FAB2878P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR3DL2/CD158k

KIR3DL2 (3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56^dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1-4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tails. Like other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid polymorphisms found in a single individual (4-6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8). It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2‑microglobulin (9, 10). NK and CD4⁺ T cells from patients with spondylarthritides show increased KIR3DL2⁺ expression and may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88-92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

References

Colonna, M. and J. Samaridis (1995) Science 268:405.
Pende, D. et al. (1996) J. Exp. Med. 184:505.
Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
Uhrberg, M. et al. (1997) Immunity 7:753.
Meenaugh, A. et al. (2004) Tiss. Antigens 64:226.
Kwon, D. et al. (2000) Mol. Cells 10:54.
Dohring, C. et al. (1996) J. Immunol. 156:3098.
Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2

Entrez Gene IDs

3812 (Human); 245615 (Mouse)

Alternate Names

killer cell immunoglobulin-like receptor 3DL2; CD158k; CL-5; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2; KIR3DL2; NKAT4; NKAT-4; NKAT4A; NKAT4B; p140

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Citations for Human KIR3DL2/CD158k PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Microenvironment-dependent growth of Sezary cells in humanized IL-15 mice
Authors: Gao, J;Ren, S;Choonoo, G;Chen, G;Frleta, D;Zhong, J;Gupta, N;Sharma, P;Oyejide, A;Atwal, GS;Macdonald, L;Murphy, A;Kuhnert, F;
Disease models & mechanisms
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients.
Authors: Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A
Mediators Inflamm, 2020-02-19;2020(0):6401969.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry