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Human Kremen-1 Antibody

Catalog #: AF2127
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2127
AF2127-SP
Kremen‑1 in Human Colon Cancer Tissue.
2 Images
Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kremen-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kremen-1 and less than 5% cross-reactivity with recombinant human Kremen-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kremen‑1
Ala20-Gly397
Accession # NP_114434
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kremen‑1
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Kremen-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Kremen‑1 in Human Colon Cancer Tissue. Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Kremen-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

Kremen‑1 in Human Colon. Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kremen-1

Kremen-1 is a type I transmembrane protein that contains extracellular Kringle, WSC, and CUB domains. It is expressed in diverse embryonic and adult tissues. Kremen proteins bind the secreted Dickkopf proteins Dkk-1, -2, and -4 with high affinity to antagonize the canonical Wnt signaling pathway.

Long Name
Kringle-containing Transmembrane Protein 1
Entrez Gene IDs
83999 (Human); 84035 (Mouse)
Alternate Names
EC 3.4.21; FLJ31863; KREMEM1; kremen protein 1; KREMEN; Kremen1; Kremen-1; kringle containing transmembrane protein 1; kringle containing transmembrane protein; Kringle domain-containing transmembrane protein 1; kringle-coding gene marking the eye and the nose; Kringle-containing protein marking the eye and the nose; KRM1; KRM1Dickkopf receptor

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Citations for Human Kremen-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A WNT4 and DKK3 driven canonical to noncanonical Wnt signaling switch controls multiciliogenesis
    Authors: Cooney, RA;Saal, ML;Geraci, KP;Maynard, C;Cleaver, O;Hoang, ON;Moore, TT;Hwang, RF;Axelrod, JD;Vladar, EK;
    Journal of cell science
    Species: Human
    Sample Types: Whole Cells, Transduced Whole Cells
    Applications: Immunocytochemistry
  2. Dickkopf proteins influence lung epithelial cell proliferation in idiopathic pulmonary fibrosis
    Authors: E.-M. Pfaff, S. Becker, A. Gunther, M. Konigshoff
    European Respiratory Journal

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