Human L-Selectin/CD62L PE-conjugated Antibody

Catalog #: FAB9787P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB9787P-100

Detection of L‑Selectin/CD62L in PBMC with CD3 costain cells by Flow Cytometry.

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Human L-Selectin/CD62L PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Binds to CHO cells expressing the human L‑Selectin/CD62L protein. It does not recognize CHO cells expressing either the human E-Selectin or human P-Selectin.

Source

Monoclonal Mouse IgG₁ Clone # 4G8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human L‑Selectin/CD62L Extracellular domain

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

PBMCs with CD3 costain or PBMCs with CD45RA costain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of L‑Selectin/CD62L in PBMC with CD3 costain cells by Flow Cytometry. PBMCs were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L PE‑conjugated Monoclonal Antibody (Catalog # FAB9787P) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of L‑Selectin/CD62L in PBMCs with CD45RA costain by Flow Cytometry. PBMCs were stained with Mouse Anti-Human CD45RA MFV610‑conjugated Monoclonal Antibody (Catalog # FAB11444MFV610) and either (A) Mouse Anti-Human L‑Selectin/CD62L PE‑conjugated Monoclonal Antibody (Catalog # FAB9787P) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: L-Selectin/CD62L

L-Selectin (LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. It plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites.

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Alternate Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1