Human Laforin/EPM2A Antibody

Catalog #: MAB5714 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5714
MAB5714-SP

Detection of Human Laforin/EPM2A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Laforin/EPM2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Laforin/EPM2A in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 523435

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Laforin/EPM2A
Met1-Leu331
Accession # AAH70047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Laforin/EPM2A by Western Blot. Western blot shows lysates of human heart and brain tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human Laforin/EPM2A Monoclonal Antibody (Catalog # MAB5714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Laforin (3 ng) was included. A specific band for Laforin/EPM2A was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laforin/EPM2A

Laforin, also known as Lafora PTPase and EPM2A, is a 38 kDa member of the protein tyrosine phosphatase family. Human Laforin is 331 aa in length and contains one carbohydrate binding type-20 (CBM20) domain (aa 1-124) and one tyrosine-protein phosphatase domain (aa 243-311). Multiple splicing variants produce four isoforms of human Laforin, which is most highly expressed in heart, skeletal muscle, kidney, pancreas and brain. It functions as a dual specificity protein phosphatase and may be involved in the control of glycogen metabolism. Mutations in Laforin cause progressive myoclonic epilepsy type 2, also known as Lafora disease.

Entrez Gene IDs

7957 (Human); 13853 (Mouse); 680683 (Rat)

Alternate Names

EC 3.1.3.16; EC 3.1.3.48; epilepsy, progressive myoclonus type 2, Lafora disease (laforin); epilepsy, progressive myoclonus type 2A, Lafora disease (laforin); EPM2; EPM2A; Lafora PTPase; Laforin; LAFPTPase; LD; LDE; MELF