Human LAMP-1/CD107a Antibody

LAMP1/CD107a in Human Kidney. LAMP1/CD107a was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

LAMP‑1/CD107a in THP‑1 Human Cell Line. LAMP-1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human LAMP-1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human LAMP-1/CD107a by Western Blot Increased SNX9 expression and co-localization with podocin are detectable in the cytoplasm of ADR-treated WT podocytes, whereas SNX9 KD podocytes exhibit little cytoplasmic expression of podocin.(a) Fluorescent micrographs of cultured human podocytes stained with SNX9 (green) and podocin (red) before and after ADR treatment (merged areas are in yellow). DAPI (blue) was used to indicate nuclei. Boxes indicate higher magnification areas presented in the lower panels. (b) Western blot analyses of the fractions from control podocytes or podocytes treated with ADR separated on linear OptiPrep gradients (5–25%). Distributions of SNX9 and podocin, as well as marker proteins of plasma membrane (caveolin), endosome/lysosome (LAMP1), mitochondria ( beta subunit of F1F0-ATPase), and endoplasmic reticulum (calnexin), were examined by western blot analysis. (c) Cultured human podocytes were transfected with nonfunctional control siRNA (upper panel) or SNX9 siRNA (middle and lower panels). Transfected cells, as decided by GFP expression, are indicated by arrowheads. Upper panel: Fluorescent micrographs of control siRNA-transfected podocyte stained with SNX9 (red). Middle panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with SNX9 (red). Lower panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with podocin (red). DAPI (blue) was used to indicate nuclei. Boxes indicate higher-magnification areas presented on the right. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28266622), licensed under a CC-BY license. Not internally tested by R&D Systems.