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Human LAMP-1/CD107a Lumenal Domain Antibody

Catalog #: AF4800
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4800
AF4800-SP
Detection of Human LAMP‑1/CD107a by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LAMP-1/CD107a Lumenal Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse LAMP1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LAMP-1/CD107a antibody by Western Blot. View Larger

Detection of Human LAMP‑1/CD107a by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LAMP1/CD107a antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

LAMP1/CD107a in THP-1 Human Cell Line. LAMP1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using 10 µg/mL Sheep Anti-Human LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

LAMP1 (Lysosome-associated membrane protein-1; also CD107a) is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs
3916 (Human); 16783 (Mouse)
Alternate Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1

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Citations for Human LAMP-1/CD107a Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Increased Immunoreactivity of Cathepsins in the Rat Esophagus under Chronic Acid Reflux Esophagitis
    Authors: Masayuki Suyama, Masato Koike, Daisuke Asaoka, Hiroki Mori, Masako Oguro, Takashi Ueno et al.
    Journal of Histochemistry & Cytochemistry
  2. An Alzheimer's linked loss-of-function CLN5 variant impairs Cathepsin D maturation consistent with a retromer trafficking defect.
    Authors: Qureshi YH, Patel VM, Berman DE et al.
    Mol. Cell. Biol.
  3. Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases
    Authors: SE Cason, ELF Holzbaur
    bioRxiv : the preprint server for biology, 2023-01-29;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  4. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy
    Authors: ER Gallagher, ELF Holzbaur
    Cell Reports, 2023-01-25;42(2):112037.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation
    Authors: Cason SE, Mogre SS, Holzbaur ELF, Koslover EF.
    Molecular Biology of the Cell
  6. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020-04-04;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  7. Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity
    Authors: AM Kolodzieje, MA Altura, J Fan, EM Petersen, M Cook, PS Brzovic, SI Miller
    Cell Rep, 2019-05-14;27(7):2147-2156.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling: role of CD14 and CD36.
    Authors: Nilsen NJ, Deininger S, Nonstad U, Skjeldal F, Husebye H, Rodionov D, von Aulock S, Hartung T, Lien E, Bakke O, Espevik T
    J. Leukoc. Biol., 2008-05-05;84(1):280-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. beta -Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway
    Authors: Sourish Ghosh, Teegan A. Dellibovi-Ragheb, Adeline Kerviel, Eowyn Pak, Qi Qiu, Matthew Fisher et al.
    Cell
  10. Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation
    Authors: Cason SE, Mogre SS, Holzbaur ELF, Koslover EF.
    Molecular Biology of the Cell

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Human LAMP-1/CD107a Lumenal Domain Antibody
By Karina Kinghorn on 08/04/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

LAMP1 (red), VEGFR2 (green), Nucleus (blue)

Immunocytochemistry/Immunofluorescence Human LAMP-1/CD107a Lumenal Domain Antibody AF4800