Home / LAMP-1/CD107a / Human LAMP-1/CD107a PE-conjugated Antibody

Human LAMP-1/CD107a PE-conjugated Antibody

Catalog #: IC4800P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC4800P
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LAMP-1/CD107a PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAMP1/CD107a in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 508921
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
Enriched or expanded human NK cells cultured with 1:1 ratio of K562 cell line target cells
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. NK cells were expanded from PBMC using Cloudz Human NK Cell Expansion Kit (CLD004) and cultured in the presence of Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P) for 2 hours either (A) with or (B) without a 1:1 ratio of K562 cell line target cells. Cells were then stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (FAB2408A). Quadrant markers were set based on control antibody staining (IC0041P). Staining was performed using our Staining Surface Molecules protocol.

Flow Cytometry Detection of LAMP-1/CD107a antibody in Human NK cells antibody by Flow Cytometry. View Larger

Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line for 5 hours or (B) untreated were stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P).

Intracellular Staining by Flow Cytometry Detection of LAMP-1/CD107a antibody in THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LAMP‑1/CD107a in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs
3916 (Human); 16783 (Mouse)
Alternate Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human LAMP-1/CD107a PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020-04-21;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human LAMP-1/CD107a PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human LAMP-1/CD107a PE-conjugated Antibody and earn rewards!

Have you used Human LAMP-1/CD107a PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review