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Human LBP Antibody

Catalog #: AF870
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF870
AF870-SP
Detection of Human LBP by Western Blot
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LBP Antibody Summary

Species Reactivity
Human
Specificity
Detects human LBP in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse LBP is observed and less than 1% cross-reactivity with recombinant human BPI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LBP
Ala26-Val481
Accession # AAD21962
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LBP (Catalog # 870-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LBP by Western Blot View Larger

Detection of Human LBP by Western Blot Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human LBP by Western Blot View Larger

Detection of Human LBP by Western Blot Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.

References
  1. Hailman, E. et al. (1994) J. Exp. Med. 179:269.
  2. Jack, R.S. et al. (1997) Nature 389:742.
  3. Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.
Long Name
Lipopolysaccharide-binding Protein
Entrez Gene IDs
3929 (Human); 16803 (Mouse)
Alternate Names
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233

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Citations for Human LBP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IL‐22 and IL‐22‐Binding Protein Are Associated With Development of and Mortality From Acute‐on‐Chronic Liver Failure
    Authors: Katharina Schwarzkopf, Sabrina Rüschenbaum, Samarpita Barat, Chengcong Cai, Marcus M. Mücke, Daniel Fitting et al.
    Hepatology Communications
  2. Quantitative proteomic analysis by iTRAQ(R) for the identification of candidate biomarkers in ovarian cancer serum
    Authors: Kristin LM Boylan, John D Andersen, Lorraine B Anderson, LeeAnn Higgins, Amy PN Skubitz
    Proteome Science
  3. Inhibition of lipid A-mediated type I interferon induction by bactericidal/permeability-increasing protein (BPI).
    Authors: Azuma M, Matsuo A, Fujimoto Y, Fukase K, Hazeki K, Hazeki O, Matsumoto M, Seya T
    Biochem. Biophys. Res. Commun., 2007-01-10;354(2):574-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe
    Authors: Gaspard Kerner, Anna-Lena Neehus, Quentin Philippot, Jonathan Bohlen, Darawan Rinchai, Nacim Kerrouche et al.
    Cell Genomics

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