Human LDLR Antibody Summary
Ala22-Arg788
Accession # P01130
Applications
Human LDLR Sandwich Immunoassay
Human LDL R Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LDLR in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B).
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Detection of LDLR in A172 cells by Flow Cytometry. A172 cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.
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Detection of LDLR in U-118-MG cells by Flow Cytometry U-118-MG cells were stained with Mouse Anti-Human LDLR Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LDLR
The Low Density Lipoprotein Receptor (LDLR) is the founding member of the LDLR family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDLR is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120-160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDLR is 51% aa identical to the extracellular region of human VLDLR, and 79% aa identical to the extracellular region of mouse LDLR. LDLR is constitutively expressed and binds ApoB of LDL and ApoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).
- Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
- Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
- Yamamoto, T. et al. (1984) Cell 39:27.
- Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
- Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
- Varret, M. et al. (2008) Clin Genet. 73:1.
Product Datasheets
Citations for Human LDLR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels
Authors: Matthew R. Zanotelli, Hamisha Ardalani, Jue Zhang, Zhonggang Hou, Eric H. Nguyen, Scott Swanson et al.
Acta Biomaterialia
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Shifts in receptors during submergence of an encephalitic arbovirus
Authors: Li, W;Plante, JA;Lin, C;Basu, H;Plung, JS;Fan, X;Boeckers, JM;Oros, J;Buck, TK;Anekal, PV;Hanson, WA;Varnum, H;Wells, A;Mann, CJ;Tjang, LV;Yang, P;Reyna, RA;Mitchell, BM;Shinde, DP;Walker, JL;Choi, SY;Brusic, V;Llopis, PM;Weaver, SC;Umemori, H;Chiu, IM;Plante, KS;Abraham, J;
Nature
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
VLDLR and ApoER2 are receptors for multiple alphaviruses
Authors: LE Clark, SA Clark, C Lin, J Liu, A Coscia, KG Nabel, P Yang, DV Neel, H Lee, V Brusic, I Stryapunin, KS Plante, AA Ahmed, F Catterucci, TL Young-Pear, IM Chiu, PM Llopis, SC Weaver, J Abraham
Nature, 2021-12-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation and Characterization of a Novel Small Biologic Alternative to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Antibodies, DS-9001a, Albumin Binding Domain-Fused Anticalin Protein
Authors: Y Masuda, S Yamaguchi, C Suzuki, T Aburatani, Y Nagano, R Miyauchi, E Suzuki, N Yamamura, K Nagatomo, H Ishihara, K Okuno, F Nara, G Matschiner, R Hashimoto, T Takahashi, T Nishizawa
J. Pharmacol. Exp. Ther., 2018-02-20;365(2):368-378.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture -
A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation
Authors: Delia Susan-Resi
J. Biol. Chem, 2016-12-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
Authors: Elodie Weider
J Biol Chem, 2016-06-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Endophilin marks and controls a clathrin-independent endocytic pathway.
Authors: Boucrot E, Ferreira A, Almeida-Souza L, Debard S, Vallis Y, Howard G, Bertot L, Sauvonnet N, McMahon H
Nature, 2014-12-17;517(7535):460-5.
Applications: Functional Assay -
Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
Blood, 2014-02-27;123(9):1422-4.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
J Allergy Clin Immunol, 2014-02-14;133(4):1227-30.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013-02-19;288(15):10805-18.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development -
Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
J. Biol. Chem., 2009-07-27;284(42):28856-64.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Multiparametric platform for profiling lipid trafficking in human leukocytes
Authors: Simon G. Pfisterer, Ivonne Brock, Kristiina Kanerva, Iryna Hlushchenko, Lassi Paavolainen, Pietari Ripatti et al.
Cell Reports Methods
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Resistin Modulates Low-Density Lipoprotein Cholesterol Uptake in Human Placental Explants via PCSK9
Authors: Sonia Nava-Salazar, Arturo Flores-Pliego, Giovanni Pérez-Martínez, Sandra Parra-Hernández, America Vanoye-Carlo, Francisco Ibarguengoitia-Ochoa et al.
Reproductive Sciences
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