Human LDLR Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of human LDL R spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 92-106 |
| Urine (n=4) | 94 | 91-99 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: LDLR
LDL R (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDL R binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface. Lack of LDL R expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human LDLR Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis
Authors: A Alabi, XD Xia, HM Gu, F Wang, SJ Deng, N Yang, A Adijiang, DN Douglas, NM Kneteman, Y Xue, L Chen, S Qin, G Wang, DW Zhang
Nature Communications, 2021-03-25;12(1):1889.
Species: Human
Sample Types: Cell Culture Supernates
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Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels
Authors: XH Liang, H Sun, W Shen, S Wang, J Yao, MT Migawa, HH Bui, SS Damle, S Riney, MJ Graham, RM Crooke, ST Crooke
Nucleic Acids Res., 2017-09-19;45(16):9528-9546.
Species: Human
Sample Types: Cell Culture Supernates
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Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
Authors: SA Felt, GN Droby, VZ Grdzelishv
J. Virol., 2017-07-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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