Human Lefty-A Long Isoform Antibody

Catalog #: MAB746 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB746
MAB746-SP

All Lefty-A Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lefty-A Long Isoform Antibody Summary

Species Reactivity

Human

Specificity

Detects the long isoform of recombinant human (rh) Lefty-A (aa 78‑366) in direct ELISAs and Western blots. Does not cross-react with the short form of recombinant human Lefty-A (aa 136‑366) or with rhART, recombinant mouse (rm) Artemin, recombinant drosophila DPP, rhGDNF, rhLAP, recombinant rat MIS, rhNeurturin, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, or rmLefty-1.

Source

Monoclonal Mouse IgG_2A Clone # 182515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lefty-A
Phe78-Pro366
Accession # O00292.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Lefty-A (Catalog # 746-LF)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty-A

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial Bleeding-Associated Factor. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and -B are more similar to each other (96%) than to either Lefty-1 or -2 in the mouse (81‑82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, Lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of Lefty are larger than mature forms of other TGF‑ beta ‑related proteins. Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of Lefty on the left side is well conserved. Furthermore its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

References

Meno, C. et al. (1996) Nature 381:151.
Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
Schier, A.F. and M.M. Shen, (1999) Nature 403:385.
Branford, W.W. et al. (2000) Dev. Biol. 223:291.
Tabibzadeh, S. et al. (2000) J. Clin. Endocr. Metab. 85:2526.
Ulloa, L. et al. (2001) J. Biol. Chem. 276:21387.

Entrez Gene IDs

7044 (Human)

Alternate Names

EBAFTGF-beta-4; endometrial bleeding associated factor; Endometrial bleeding-associated factor; Lefta; LEFTAProtein lefty-A; left-right determination factor 2; Left-right determination factor A; LEFTY2; LeftyA; Lefty-A; LEFTYATransforming growth factor beta-4; MGC46222; Protein lefty-2; TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)