Human LIM1 Antibody Summary
Leu265-Trp406
Accession # P48742
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
LIM1 in Human Brain (Cerebellum). LIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human LIM1 Monoclonal Antibody (Catalog # MAB2725) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIM1
LIM homeobox 1 gene product (LIM1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM1. A truncated human isoform does not include this sequence. LIM1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization.
Product Datasheets
Citations for Human LIM1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
LIM1 contributes to the malignant potential of endometrial cancer
Authors: Hiroaki Kato, Noritaka Saeki, Matome Imai, Hiroshi Onji, Akiko Yano, Shuhei Yoshida et al.
Frontiers in Oncology
-
Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
Cell Rep, 2020-04-07;31(1):107471.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling
Authors: Xin Huang, Sophie Balmer, Fan Yang, Miguel Fidalgo, Dan Li, Diana Guallar et al.
eLife
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human LIM1 Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human LIM1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
