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Human LIN-41 Antibody

Catalog #: AF5104
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5104
AF5104-SP
Detection of Human LIN‑41 by Western Blot.
3 Images
Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIN-41 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIN-41 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LIN-41
Val386-Phe868
Accession # Q2Q1W2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LIN‑41 antibody by Western Blot. View Larger

Detection of Human LIN‑41 by Western Blot. Western blot shows lysates of BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN-41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry LIN-41 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

LIN‑41 in BG01V Human Stem Cells. LIN-41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human LIN-41 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human LIN‑41 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN-41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIN-41

LIN-41 (lineage-41; also TRIM/tripartite motif-containing protein 71) is a 94 kDa (predicted) cytosolic member of the RBCC (RING finger, B-Box Coiled-Coil) family of proteins. It is expressed in developing limbs, DRG and branchial arches and apparently regulates the timed expression of proliferation and differentiation-associated genes. Human LIN-41 is 868 amino acids (aa) in length. It contains a RING-type zinc finger region (aa 12-95), a His-rich segment (aa 146‑157), two B Box zinc fingers that bind DNA (aa 194-314), a coiled-coil motif that binds protein (aa 391-427), one filamin repeat and six NHL repeats that regulate gene translation (aa 593‑868). Over aa 386-868, human LIN-41 is 97%, 95%, and 94% aa identical to canine, mouse, and chicken LIN-41, respectively.

Long Name
RNA-binding Protein LIN-41
Entrez Gene IDs
131405 (Human); 636931 (Mouse); 301042 (Rat)
Alternate Names
LIN41; LIN-41; TRIM71; tripartite motif containing 71; tripartite motif-containing protein 71

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Citations for Human LIN-41 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. hnRNP Q/SYNCRIP interacts with LIN28B and modulates the LIN28B/let-7 axis in human hepatoma cells
    Authors: Chang, JJ;Lin, T;Jhang, XY;Chan, SP;
    PloS one
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
    Authors: Bronevetsky, Yelena, Villarino, Alejandr, Eisley, Christop, Barbeau, Rebecca, Barczak, Andrea J, Heinz, Gitta A, Kremmer, Elisabet, Heissmeyer, Vigo, McManus, Michael, Erle, David J, Rao, Anjana, Ansel, K Mark
    J Exp Med, 2013-02-04;210(2):417-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Repressing Ago2 mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAs
    Authors: Qiuying Liu, Xiaoli Chen, Mariah K Novak, Shaojie Zhang, Wenqian Hu
    eLife

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