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Human LINGO-1 Antibody

Catalog #: AF3086
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3086
AF3086-SP
LINGO‑1 in Mouse Spinal Cord.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LINGO-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human LINGO-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LINGO-1
Thr40-Thr556
Accession # NP_116197
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LINGO‑1
Immunohistochemistry
1.7-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry LINGO-1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

LINGO‑1 in Mouse Spinal Cord. LINGO-1 was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human LINGO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3086) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LINGO-1

LINGO-1 (LRRN6A) is a 614 aa transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa identity between human, mouse and rat.

Long Name
Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1
Entrez Gene IDs
84894 (Human)
Alternate Names
FLJ14594; LERN1; LERN1UNQ201; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LINGO1; LINGO-1; LRRN6A; LRRN6Aleucine-rich repeat neuronal 6A; MGC17422

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Citations for Human LINGO-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. LINGO family receptors are differentially expressed in the mouse brain and form native multimeric complexes
    Authors: Anthony Guillemain, Yousra Laouarem, Laetitia Cobret, Dora Štefok, Wanyin Chen, Solal Bloch et al.
    The FASEB Journal
  2. Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
    Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
    J. Invest. Dermatol., 2010-02-11;130(6):1685-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
    Authors: Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
    J. Neurosci., 2008-03-12;28(11):2753-65.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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