Human Lipin 1 Biotinylated Antibody

Catalog #: BAF3885 Datasheet / COA / SDS

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BAF3885

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Human Lipin 1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lipin 1 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Lipin 1

Immunocytochemistry

5-15 µg/mL

Immersion fixed SK-Mel-28 human malignant melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Lipin 1 in SK‑Mel‑28 Human Cell Line. Lipin 1 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human Lipin 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3885) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 1

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.

Long Name

Phosphatidate Phosphatase LPIN1

Entrez Gene IDs

23175 (Human); 14245 (Mouse); 313977 (Rat)

Alternate Names

DKFZp781P1796; EC 3.1.3.18; EC 3.1.3.4; EC 5.4.2.7; KIAA0188phosphatidate phosphatase LPIN1; Lipin 1; lipin-1; LPIN1; PAP1