Human Livin Biotinylated Antibody

Name: Human Livin Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1161

Catalog #: BAF1161 Datasheet / COA / SDS

Discontinued Product

BAF1161 has been discontinued.

View all Livin products.

Detection of Human Livin by Western Blot.

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Human Livin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant and endogenous human Livin alpha (KIAP) and Livin beta (ML-IAP) in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Livin beta
Met1-Gln280
Accession # Q96CA5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Livin by Western Blot. Western blot shows lysates of G361 human melanoma cell line and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human Livin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Livin at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% bovine serum albumin.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Livin

Livin alpha (KIAP) and Livin beta (ML-IAP) are two splice forms generated from the livin gene. Livin is a member of the inhibitor of apoptosis protein (IAP) family that contains a baculovirus single IAP repeat (BIR) and a C-terminal RING finger domain. Livin inhibits the activation of caspases 9 and/or 3 when activated in specific cell lines. Livin alpha is a splice variant 18 amino acids longer in the BIR-RING linking region than the livin beta splice variant. The isoforms differ in their apoptotic properties.

Entrez Gene IDs

79444 (Human)

Alternate Names

baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; BIRC7; KIAP; KIAPRING finger protein 50; Kidney inhibitor of apoptosis protein; livin inhibitor-of-apoptosis; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAPlivin inhibitor of apoptosis; ML-IAPmliap; RNF50LIVINbaculoviral IAP repeat-containing protein 7