Human LMO2 Antibody

Catalog #: AF2726
15 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2726
AF2726-SP

Detection of LMO2 in K562 Human Cell Line by Flow Cytometry.

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All LMO2 Antibodies

Product Details

Citations (15)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LMO2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LMO2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LMO2

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LMO2 in K562 Human Cell Line by Flow Cytometry. K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of LMO2 in Human PBMC by Flow Cytometry Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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LMO2 in K562 Human Cell Line. LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot

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Detection of Mouse LMO2 by Western Blot Gene expression analysis of Tal1−/− Flk-1+ cells. (A) Lmo2, Tal1 gene expression relative to Hprt in Flk-1+ cells derived from WT, Lmo2−/− or Tal1−/− ES cells. Data represent the mean of three independent samples measured in duplicate ± standard deviation. (B) LMO2, TAL1, LDB1 and beta -actin protein levels detected by western blotting using nuclear extract from WT, Lmo2−/− and Tal1−/− Flk-1+ cells. (C) Pairwise comparison of significantly differentially expressed genes. Quantitation and analyses of RNAseq data are based on biological triplicate samples. (D) Heat map showing hierarchical clustering of differentially expressed genes at the Flk-1+ stage. (E) Gene ontology enrichment analysis for biological process was performed on cluster 5 as identified in D. Terms were ordered according to their Modified Fisher Extract P-value and only terms with P < 0.05 were considered significant. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/17/9874/3902958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Entrez Gene IDs

4005 (Human)

Alternate Names

Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO2; LMO-2; RBTN2; RBTN2rhombotin-2; RBTNL1; RBTNL1rhombotin-like 1; RHOM2; RHOM2T-cell translocation protein 2; T-cell translocation gene 2; TTG2; TTG2rhombotin 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human LMO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Inhibition of G9a methyltransferase stimulates fetal hemoglobin production by facilitating LCR/gamma-globin looping.
Authors: Krivega I, Byrnes C, de Vasconcellos J, Lee Y, Kaushal M, Dean A, Miller J
Blood, 2015-05-15;126(5):665-72.
SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia
Authors: Courtney Chambers, Katerina Cermakova, Yuen San Chan, Kristen Kurtz, Katharina Wohlan, Andrew Henry Lewis et al.
Cancer Research
LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice
Authors: KI Hirano, H Hosokawa, M Koizumi, Y Endo, T Yahata, K Ando, K Hozumi
Elife, 2021-08-12;10(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP
A cell-based screening method using an intracellular antibody for discovering small molecules targeting the translocation protein LMO2
Authors: N Bery, CJR Bataille, A Russell, A Hayes, F Raynaud, S Milhas, S Anand, H Tulmin, A Miller, TH Rabbitts
Science Advances, 2021-04-09;7(15):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Ldb1 is required for Lmo2 oncogene–induced thymocyte self-renewal and T-cell acute lymphoblastic leukemia
Authors: LiQi Li, Apratim Mitra, Kairong Cui, Bin Zhao, Seeyoung Choi, Jan Y. Lee et al.
Blood
Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells
Authors: M Nafria, P Keane, ES Ng, EG Stanley, AG Elefanty, C Bonifer
Cell Rep, 2020-05-26;31(8):107691.
Species: Human
Sample Types:
Applications: ChIP-Seq
LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage
Authors: VS Stanulovic, P Cauchy, SA Assi, M Hoogenkamp
Nucleic Acids Res., 2017-09-29;45(17):9874-9888.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
Nat Commun, 2016-11-21;7(0):13396.
Species: Human
Sample Types: Complex Sample Type
Original Research: Generation of non-deletional hereditary persistence of fetal hemoglobin beta -globin locus yeast artificial chromosome transgenic mouse models: -175 Black HPFH and -195 Brazilian HPFH
Authors: Carolina A Braghini, Flavia C Costa, Halyna Fedosyuk, Renee Y Neades, Lesya V Novikova, Matthew P Parker et al.
Experimental Biology and Medicine
Editing the genome to introduce a beneficial naturally occurring mutation associated with increased fetal globin
Authors: Beeke Wienert, Alister P. W. Funnell, Laura J. Norton, Richard C. M. Pearson, Lorna E. Wilkinson-White, Krystal Lester et al.
Nature Communications

Identification of a Dynamic Core Transcriptional Network in t(8;21) AML that Regulates Differentiation Block and Self-Renewal
Authors: Anetta Ptasinska, Salam A. Assi, Natalia Martinez-Soria, Maria Rosaria Imperato, Jason Piper, Pierre Cauchy et al.
Cell Reports

The hematopoietic regulator TAL1 is required for chromatin looping between the beta -globin LCR and human gamma -globin genes to activate transcription
Authors: Won Ju Yun, Yea Woon Kim, Yujin Kang, Jungbae Lee, Ann Dean, AeRi Kim
Nucleic Acids Research

Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential.
Authors: Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B
J Exp Med, 2014-03-31;211(4):701-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19).
Authors: Hirose K, Inukai T, Kikuchi J, Furukawa Y, Ikawa T, Kawamoto H, Oram SH, Gottgens B, Kiyokawa N, Miyagawa Y, Okita H, Akahane K, Zhang X, Kuroda I, Honna H, Kagami K, Goi K, Kurosawa H, Look AT, Matsui H, Inaba T, Sugita K
Blood, 2010-06-02;116(6):962-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

GFI1B controls its own expression binding to multiple sites
Authors: Eduardo Anguita, Ana Villegas, Francisco Iborra, Aurora Hernandez
Haematologica