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Human LPAR1/LPA1/EDG-2 Antibody

Catalog #: MAB9963 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9963-100
MAB9963-SP
LPAR1/LPA1/EDG-2 in Human Prostate Cancer Tissue.
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Human LPAR1/LPA1/EDG-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LPAR1/LPA1/EDG-2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 999807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LPAR1/LPA1/EDG-2
Met1-Lys50
Accession # Q92633
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry LPAR1/LPA<sub>1</sub>/EDG-2 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

LPAR1/LPA1/EDG-2 in Human Prostate Cancer Tissue. LPAR1/LPA1/EDG-2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB9963) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name
Lysophosphatidic Acid Receptor 1
Entrez Gene IDs
1902 (Human); 14745 (Mouse)
Alternate Names
EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1

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Human LPAR1/LPA1/EDG-2 Antibody
By Anonymous on 10/14/2022
Application: IHC Sample Tested: Prostate tissue Species: Human
Immunohistochemistry Human LPAR1/LPA1/EDG-2 Antibody MAB9963