Human LPAR1/LPA₁/EDG-2 Antibody

Catalog #: AF9963
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF9963-100
AF9963-SP

Detection of Human LPAR1/LPA1/EDG-2 by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LPAR1/LPA₁/EDG-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LPAR-1/LPA₁/EDG-2 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LPAR-1 CD33 protein containing the sequences for the four extracellular domains, amino acids (aa) 1-50, aa112-125, aa190-205 and aa281-294
Accession # Q92633

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LPAR1/LPA₁/EDG-2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human brain (cerebellum) tissue, and human liver tissue (negative control). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LPAR1/LPA₁/EDG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9963) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LPAR1/LPA₁/EDG-2 at approximately 48-51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name

Lysophosphatidic Acid Receptor 1

Entrez Gene IDs

1902 (Human); 14745 (Mouse)

Alternate Names

EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human LPAR1/LPA₁/EDG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
Cancer Cell Int, 2020-10-02;20(0):476.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot