Human LRIG2 Antibody

Name: Human LRIG2 Antibody
Brand: R&D Systems
SKU: MAB19411
Rating: 5 (1 reviews)

Catalog #: MAB19411 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19411-100
MAB19411-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRIG2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRIG2 in immunocytochemistry and immunohistochemistry.

Source

Monoclonal Mouse IgG₁ Clone # 990332

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
Gly41-Thr805
Accession # O94898

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1.7-25 µg/mL

See below

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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LRIG2 in WM‑115 Human Cell Line. LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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LRIG2 in Human Kidney. LRIG2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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LRIG2 in Human Squamous Cell Carcinoma. LRIG2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB19411) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRIG2

LRIG2 (Leucine-rich Repeats and Ig-like domains-2; also LIG-2) is an approximately 119-132 kDa glycoprotein that belongs to the LRIG gene family. The LRIG2 protein was predicted to have the same domain organization as LRIG1with a signal peptide, an extracellular part containing 15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. Over aa 41‑805, human LRIG2 shares 94%, 93% and 92% aa sequence identity with mouse, rat and macaque LRIG2 respectively.

Long Name

Leucine-rich Repeats and Immunoglobulin-like Domains 2

Entrez Gene IDs

9860 (Human); 269473 (Mouse); 310753 (Rat)

Alternate Names

KIAA0806; leucine-rich repeats and immunoglobulin-like domains 2; leucine-rich repeats and immunoglobulin-like domains protein 2; LIG2; LRIG2; UFS2