Human LRP-4 Antibody

Catalog #: MAB5948
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5948
MAB5948-SP

Detection of Human and Rat LRP‑4 by Western Blot.

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All LRP-4 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRP-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRP-5 or rhLRP-6 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 741704

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LRP-4
Gly18-Ser344
Accession # O75096

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat LRP‑4 by Western Blot. Western blot shows lysates of rat embryonic cortical glial cells and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of LRP‑4 in U-2 OS Human Cell Line by Flow Cytometry. U-2 OS human osteosarcoma cell line was stained with Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948, filled histo-gram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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LRP‑4 in Human Brain. LRP-4 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-4

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells and binds multiple ligands including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 97% aa identity with both mouse and rat LRP‑4. MAB5948 detects the correct rat band in Western Blot. Immunohistochemistry was not tested in rat samples.

Long Name

LDL Receptor-related Protein 4

Entrez Gene IDs

4038 (Human); 228357 (Mouse); 83469 (Rat)

Alternate Names

CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP4; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7

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Citation for Human LRP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1