Human LRRTM3 Antibody

Catalog #: MAB4898 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4898
MAB4898-SP

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All LRRTM3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRRTM3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRRTM3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) LRRTM3 and no cross-reactivity with recombinant human LRRTM1, 2, or 4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 568318

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRTM3
Glu31-Lys419
Accession # Q86VH5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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LRRTM3 in Human Brain. LRRTM3 was detected in immersion fixed paraffin-embedded sections of human brain with Alzheimer's disease using Human LRRTM3 Monoclonal Antibody (Catalog # MAB4898) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRTM3

Human LRRTM3 (leucine-rich repeat transmembrane neuronal 3) is a 63 kDa (predicted) type I transmembrane glycoprotein that is one of four LRRTM family members within the LRR protein superfamily (1). The LRRTM3 cDNA encodes 581 amino acids (aa), including a 389 aa extracellular region that shares 98% aa identity with mouse and rat LRRTM3. Isoform 2 lacks cytoplasmic aa 514-581. LRRTMs in mouse show distinct expression patterns during formation of the central nervous system (CNS) and expression persists in adult brain. LRRTM3 promotes processing of amyloid precursor protein by BACE1, and may be mutated in late-onset Alzheimer’s disease.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 3

Entrez Gene IDs

347731 (Human); 216028 (Mouse); 294380 (Rat)

Alternate Names

leucine rich repeat transmembrane neuronal 3; leucine-rich repeat transmembrane neuronal protein 3; LRRTM3; MGC131810