Human Lymphotoxin-alpha /TNF-beta Antibody

Catalog #: AB-211-NA Datasheet / COA / SDS

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AB-211-NA

Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutralization by Human Lymphotoxin‑ alpha /TNF‑ beta Antibody.

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All Lymphotoxin-alpha/TNF-beta Antibodies

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Human Lymphotoxin-alpha /TNF-beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TNF-alpha and recombinant mouse TNF-alpha is observed. Neutralizes the biological activity of recombinant human (rh) TNF-beta and natural human TNF-beta, but will not neutralize the biological activity of rhTNF-alpha or recombinant mouse TNF-alpha.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
Leu35-Leu205
Accession # P01374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211-TB)

Neutralization

Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.2-0.4 µg/mL in the presence of 1 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutralization by Human Lymphotoxin‑ alpha /TNF‑ beta Antibody. Recombinant Human Lymphotoxin-a/TNF-beta (Catalog # 211-TB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-a/TNF-beta (1 ng/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Human Lymphotoxin-a/TNF-beta Polyclonal Antibody (Catalog # AB-211-NA). The ND₅₀ is typically 0.2-0.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B, and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

Long Name

Tumor Necrosis Factor beta

Entrez Gene IDs

4049 (Human); 16992 (Mouse)

Alternate Names

LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1