Human Lymphotoxin-alpha /TNF-beta Antibody

Catalog #: AF-211-NA
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-211-NA
AF-211-SP

Cytotoxicity Induced by Lymphotoxin‑alpha/TNF‑beta and Neutralization by Human Lymphotoxin‑alpha/TNF‑beta Antibody.

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All Lymphotoxin-alpha/TNF-beta Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lymphotoxin-alpha /TNF-beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
Leu35-Leu205
Accession # P01374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211-TB)

Neutralization

Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221]. The Neutralization Dose (ND₅₀) is typically <200 ng/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cytotoxicity Induced by Lymphotoxin‑alpha/TNF‑beta and Neutralization by Human Lymphotoxin‑alpha/TNF‑beta Antibody. Recombinant Human Lymphotoxin-a/TNF-beta (Catalog # 211-TB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-a/TNF-beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Lymphotoxin-a/TNF-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-211-NA). The ND₅₀ is typically <200 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B, and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

Long Name

Tumor Necrosis Factor beta

Entrez Gene IDs

4049 (Human); 16992 (Mouse)

Alternate Names

LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

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Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Lymphotoxin-alpha /TNF-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Lymphotoxin-alphabeta heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis.
Authors: Young J, Yu X, Wolslegel K
Cytokine, 2010-03-31;51(1):78-86.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Development, Immunoprecipitation
Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
Authors: Casazza S, Zappia E, Pistorio A, Gambini C
Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P