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Human M-Cadherin/Cadherin-15 Antibody

Catalog #: AF4096
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4096
AF4096-SP
Detection of Human M-Cadherin/Cadherin-15 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human M-Cadherin/Cadherin-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human M‑Cadherin/Cadherin‑15 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human M-Cadherin/Cadherin-15
Val22-Ala606
Accession # P55291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human M-Cadherin/Cadherin-15 antibody by Western Blot. View Larger

Detection of Human M-Cadherin/Cadherin-15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M-Cadherin/Cadherin-15 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of M-Cadherin/ Cadherin-15 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of M‑Cadherin/ Cadherin‑15 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Sheep Anti-Human M-Cadherin/ Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunocytochemistry M-Cadherin/Cadherin-15 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

M‑Cadherin/Cadherin-15 in C2C12 Mouse Cell Line. M-Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-Cadherin/Cadherin-15

M-Cadherin (M-CAD or Cadherin-15) is a 124 kDa type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules (1-4). Like other classical Cadherins, the 814 amino acid (aa) human M-CAD contains a signal sequence (21 aa), a propeptide (29 aa), an extracellular domain with five Cadherin domain repeats (ECD, 556 aa), a transmembrane segment (20 aa) and a cytoplasmic domain (188 aa) (1). A splice variant that diverges within the third Cadherin repeat has been sequenced. The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells (1-4). Intracellularly, M-CAD binds beta -catenin or plakoglobin ( gamma -catenin), which in turn bind alpha -catenin (4). M-CAD also binds p120 catenin (5). Connection of Cadherin/catenin complexes to the actin cytoskeleton is in question, but is possible through a linker (6). Connection to microtubules has been shown (7). M-CAD is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion (8). It is also present in muscle satellite cells and participates in muscle regeneration (9). M-CAD is also expressed in the granule cell layer of the cerebellar glomerulus (10). Deletion of mouse M-CAD has little effect in vivo, most likely due to compensation by N-Cadherin (11). However, M-CAD upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion (8, 12, 13). M-CAD activity is later downregulated by sequestering to caveoli, p120 catenin/RhoA-induced ubiquitination and/or cleavage by calpain-3. This terminates fusion and allows sarcomere formation (5, 12, 13). Human M-CAD ECD shows 88% aa identity with mouse, rat, or bovine and 85% aa identity with canine M-CAD ECD. M-CAD is an outlier among classical Cadherins, with 40% aa identity or less in the ECD (4).

References
  1. Kaufmann, U. et al. (1999) Cell Tissue Res. 296:191.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Shimoyama, Y. et al. (1998) J. Biol. Chem. 273:10011.
  4. Kuch, C. et al. (1997) Exp. Cell Res. 232:331.
  5. Charasse, S. et al. (2006) Mol. Biol. Cell 17:749.
  6. Weis, W.I. and W. J. Nelson (2006) J. Biol. Chem. 281:35593.
  7. Kaufmann, U. et al. (1999) J. Cell Sci. 112:55.
  8. Zeschnigk, M. et al. (1995) J. Cell Sci. 108:2973.
  9. Wrobel, E. et al. (2007) Eur. J. Cell Biol. Jan 10; [Epub ahead of print]
  10. Rose, O. et al. (1995) Proc. Natl. Acad. Sci. USA 92:6022.
  11. Hollnagel, A. et al. (2002) Mol. Cell. Biol. 22:4760.
  12. Volonte, D. et al. (2003) Mol. Biol. Cell 14:4075.
  13. Kramerova, I. et al. (2006) Mol. Cell. Biol. 26:8437.
Long Name
M-Cadherin [Myotubule]
Entrez Gene IDs
1013 (Human); 12555 (Mouse); 361432 (Rat)
Alternate Names
cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin

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Citations for Human M-Cadherin/Cadherin-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells
    Authors: H Xi, J Langerman, S Sabri, P Chien, CS Young, S Younesi, M Hicks, K Gonzalez, W Fujiwara, J Marzi, S Liebscher, M Spencer, B Van Handel, D Evseenko, K Schenke-La, K Plath, AD Pyle
    Cell Stem Cell, 2020-05-11;0(0):.
  2. Interleukin-1beta (IL-1?)-induced Notch ligand Jagged1 suppresses mitogenic action of IL-1? on human dystrophic myogenic cells
    Authors: Y Nagata, T Kiyono, K Okamura, YI Goto, M Matsuo, M Ikemoto-Ue, N Hashimoto
    PLoS ONE, 2017-12-01;12(12):e0188821.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 2015-04-20;21(5):467-75.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC-Fr, Western Blot

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