Human M-CSF R/CD115 Antibody

Catalog #: MAB329
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB329
MAB329-SP

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Citations (6)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human M-CSF R/CD115 Antibody Summary

Species Reactivity

Human

Specificity

Detects human M-CSF R/CD115 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) GM-CSF R alpha or
rhGM-CSF R beta.

Source

Monoclonal Mouse IgG₁ Clone # 61708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115
Ile20-Glu512 (Pro54Ala)
Accession # P07333.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1‑4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References

deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
Rovida, E. et al. (2001) J. Immunol. 166:1583.
Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
Dai, X. et al. (2002) Blood 99:111.
Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
Li, M. et al. (2004) J. Neurochem. 91:623.
Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Long Name

Macrophage Colony Stimulating Factor Receptor

Entrez Gene IDs

1436 (Human); 12978 (Mouse)

Alternate Names

CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human M-CSF R/CD115 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders
Authors: Dorion, MF;Casas, D;Shlaifer, I;Yaqubi, M;Fleming, P;Karpilovsky, N;Chen, CX;Nicouleau, M;Piscopo, VEC;MacDougall, EJ;Alluli, A;Goldsmith, TM;Schneider, A;Dorion, S;Aprahamian, N;MacDonald, A;Thomas, RA;Dudley, RWR;Hall, JA;Fon, EA;Antel, JP;Stratton, JA;Durcan, TM;La Piana, R;Healy, LM;
Molecular neurodegeneration
Species: Human
Sample Types: Whole Cells, Transfected Whole Cells
Applications: Flow Cytometry
Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
J. Immunol., 2017-07-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
Authors: Lee B, Kim TH, Jun JB, Yoo DH, Woo JH, Choi SJ, Lee YH, Song GG, Sohn J, Park-Min KH, Ivashkiv LB, Ji JD
J. Immunol., 2010-10-08;185(10):5926-34.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
Leuk. Res., 2008-03-04;32(8):1244-58.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007-04-19;25(8):1862-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Serotonin 5-HT2C Receptor Cys23Ser Single Nucleotide Polymorphism Associates with Receptor Function and Localization In Vitro
Authors: Michelle A. Land, Holly L. Chapman, Brionna D. Davis-Reyes, Daniel E. Felsing, John A. Allen, F. Gerard Moeller et al.
Scientific Reports