Human MafF Antibody

Catalog #: AF3917
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3917
AF3917-SP

Detection of Human MafF by Western Blot.

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All MafF Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MafF Antibody Summary

Species Reactivity

Human

Specificity

Detects human MafF in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) MafG and rhMafK is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MafF
Ser2-Ser164
Accession # Q9ULX9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MafF by Western Blot. Western blot shows recombinant human (rh) MafF, MafG, and MafK (2 ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MafF was detected at approxi-mately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human MafF by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MafF was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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MafF in HepG2 Human Cell Line. MafF was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafF

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including
c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Small Maf/Nrf2 heterodimers have been implicated in the regulation of antioxidant response element-dependent genes.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F

Entrez Gene IDs

23764 (Human); 17133 (Mouse)

Alternate Names

hMafF; MafF; transcription factor MafF; U-Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F; v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Basic Leucine Zipper (bZIP) Transcription Factors

Citation for Human MafF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1