Human MASTL Antibody

Catalog #: AF10022 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF10022-100
AF10022-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MASTL Antibody Summary

Species Reactivity

Human

Specificity

Detects human MASTL in direct ELISA.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MASTL.
Lys39-Lys158
Accession # Q96GX5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MASTL in HeLa Human Cell Line. MASTL was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human MASTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10022) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MASTL

MAST-L is a ubiquitous microtubule associated serine/threonine kinase which plays a key role in mitosis. During entry into mitosis, CDK1 and MASTL kinase repress the activity of mitotic protein phosphatases PP1 and PP2A. Mitotic exit requires partial dephosphorylation of MASTL by PP1, followed by dephosphorylation of PP2A. PP1 is partially reactivated by decreases in CDK1 activity. PP1 then reactivates PP1A. Mutations of MASTL have been associated with autosomal dominant thrombocytopenia, a decrease in the number of platelets circulating in blood. MASTL kinase is frequently upregulated in several cancers, and is correlated with cancer progression, poor patient survival and tumor recurrence. Ectopic expression of MASTL in tumor cells has been shown to promote cell proliferation, and MASTL has been identified as a target for therapeutic intervention.

Long Name

MASTL

Entrez Gene IDs

84930 (Human); 67121 (Mouse)

Alternate Names

FLJ14813EC 2.7.11.1; Greatwall; GW; GWL; hGWL; MASTL; MAST-L; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2; THC2; THC2greatwall protein kinase