Human MBL Antibody

Catalog #: MAB23071
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB23071
MAB23071-SP

All MBL Antibodies

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Citations (1)

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Human MBL Antibody Summary

Species Reactivity

Human

Specificity

Detects human MBL in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse MBL-2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 285623

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-1.5 µg/mL of this antibody will block 50% of the binding of 8 μg/mL of Recombinant Human MBL (Catalog # 2307-MB) to immobilized mannan coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL

Human mannose/mannan-binding lectin (MBL; gene name MBL2; also called MBP-C) is a 25 kDa member of the collectin family of pattern-recognition molecules (1‑3). It is a secreted glycoprotein that is synthesized as a 248 amino acid (aa) precursor that contains a 20 aa signal sequence, a 21 aa cysteine-rich region (with three cysteines) a 58 aa collagen-like segment and a 111 aa C-type lectin domain that binds to neutral bacterial carbohydrates (3, 4). The molecule is O-glycosylated and contains multiple hydroxylated prolines and lysines (3, 5). Functionally, the molecule operates as a multimer/oligomer. The basic structural unit is a homotrimer. The homotrimer is created by the formation of interchain disulfide bonds among the cysteine-rich regions, plus a helical interaction of the collagen-like domains of each participating polypeptide (5). Mutations in the collagen region are known to interfere with proper trimer and subsequent oligomer formation (6). Once formed, the trimer, as a unit, oligomerizes with other trimers to form high molecular weight complexes. Although the exact nature of these complexes are unclear, it would appear that a three trimer complex (230 kDa) and a four trimer complex (305 kDa) constitute much of the circulating MBL (7). It is within the context of these oligomers that MBL performs its functions. After secretion by hepatocytes, oligomerized MBL will both associate with serine proteases (MASP-1, 2 & 3) and bind to bacterial carbohydrates. If the MBL complex is small, opsonization of bacreria occurs. If the complex is large, the MASPs are engaged and a complement attack complex is generated, destroying bound bacteria (3, 7, 8). Human MBL shares 63%, 61% and 65% aa identity with mouse, porcine and bovine MBL, respectively.

References

Gadjeva, M. et al. (2004) Mol. Immunol. 41:113.
Kilpatrick, D.C. (2003) Biochem. Soc. Trans. 31:745.
Presanis, J.S. et al. (2003) Biochem. Soc. Trans. 31:748.
Sastry, K. et al. (1989) J. Exp. Med. 170:1175.
Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
Larsen, F. et al. (2004) J. Biol. Chem. 279:21302.
Teillet, F. et al. (2005) J. Immunol. 174:2870.
Terai, I. et al. (2003) Eur. J. Immunol. 33:2755.

Long Name

Mannose Binding Lectin

Entrez Gene IDs

4153 (Human)

Alternate Names

COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833

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Citation for Human MBL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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