Human MD-2 Antibody

Name: Human MD-2 Antibody
Brand: R&D Systems
SKU: MAB1787
Rating: 4 (1 reviews)

Catalog #: MAB1787
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1787
MAB1787-SP

All MD2 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MD-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MD-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human MD-1.

Source

Monoclonal Mouse IgG₁ Clone # 288307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MD-2
Glu17-Asn160
Accession # Q9Y6Y9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MD‑2 (Catalog # 1787-MD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MD2

MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that associates with the extracellular domain of TLR4. MD-2 is required for LPS binding and subsequent TLR4 signaling.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Entrez Gene IDs

23643 (Human); 17087 (Mouse); 102118398 (Cynomolgus Monkey)

Alternate Names

ESOP1; ESOP-1; LY96; ly-96; lymphocyte antigen 96; MD2; MD-2; myeloid differentiation protein-2; Protein MD-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MD-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14(+) Monocytes
Authors: JM Horvatinov, EW Grogan, M Norris, A Steinkasse, H Lemos, AL Mellor, IY Tcherepano, CA Nicolette, MA DeBenedett
J. Immunol, 2017-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
Authors: Macagno A, Molteni M, Rinaldi A
J. Exp. Med., 2006-05-22;203(6):1481-92.
Species: Human
Sample Types: Buffer, Cell Lysates
Applications: ELISA Development, Western Blot