Human MEKK3 Antibody

Name: Human MEKK3 Antibody
Brand: R&D Systems
SKU: MAB6095
Rating: 4 (4 reviews)

Catalog #: MAB6095 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6095
MAB6095-SP

Detection of Human MEKK3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MEKK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MEKK3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 576240

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MEKK3
Ser43-Tyr275
Accession # Q99759

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Knockout Validated

MEKK3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in MEKK3 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MEKK3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK3 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human MEKK3 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human MEKK3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MEKK3 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MEKK3 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEKK3

MAPK/ERK kinase kinase 3 (MEKK3), also known as MEK kinase 3 and MAP3K3, is a widely expressed 71 kDa Ser/Thr kinase in the SEK, MEK, and MyD88‑IRAK‑TRAF6 signal transduction pathways. It contains a PB1 domain (aa 45‑123), a region with several phosphorylation sites, and a protein kinase domain (aa 361‑621). MEKK3 is involved in tissue morphogenesis, inflammation, and the response to cellular stress. Its activation leads to the activation of NF kappa B following stimulation of IL-1 R, TLR4, TLR8, TNF RI, and the AT1 angiotensin receptor.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 3

Entrez Gene IDs

4215 (Human); 26406 (Mouse); 303604 (Rat)

Alternate Names

EC 2.7.11.25; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MAPKKK3MEKK 3; MEK kinase 3; MEKK3; mitogen-activated protein kinase kinase kinase 3