Human Menin Antibody

Catalog #: AF6005 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6005
AF6005-SP

Detection of Human Menin by Western Blot.

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Human Menin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Menin in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Menin
aa 555-615
Accession # O00255

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Menin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Menin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Menin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Menin

Menin (Multiple endocrine neoplasia type I) is a 75‑78 kDa tumor‑suppressor protein that has no apparent structural relationship to any known protein family. It is ubiquitously expressed and interacts with other molecules, including NF kappa B, FANCD2 and ASK. Menin appears to block gene transcription, promote DNA repair, and inhibit cell proliferation. Human Menin is 615 amino acids (aa) in length. Consistent with its unique molecular taxonomy, it contains no definitive structural module(s). However, it is known to be phosphorylated on Ser399 and Thr599. There are a number of potential splice variants. Individually or in combination, there are deletions of aa 89‑95, 149‑153, 171‑173, 189‑223, and 423‑426. There is also a 50 aa substitution for aa 68‑615, a 66 aa substitution for aa 383‑615, and a two aa substitution for aa 507‑536. Over aa 555‑615, human and mouse Menin are completely identical in aa sequence.

Long Name

Multiple Endocrine Neoplasia I

Entrez Gene IDs

4221 (Human); 17283 (Mouse); 29417 (Rat)

Alternate Names

MEAI; MEN1; Menin; multiple endocrine neoplasia I; multiple