Human mGluR5 Antibody

Catalog #: MAB4514 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4514
MAB4514-SP

Detection of mGluR5 in MG‑63 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human mGluR5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human mGluR1, R2, R3, R4, R7, or R8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 464818

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human mGluR5
Gln21-Ser509
Accession # P41594

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human mGluR5

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of mGluR5 antibody in MG‑63 Human Cell Line antibody by Flow Cytometry.

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Detection of mGluR5 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB4514, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR5

Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 aa in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca⁺⁺-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat, and dog mGluR5.

Long Name

Metabotropic Glutamate Receptor 5

Entrez Gene IDs

2915 (Human); 108071 (Mouse); 24418 (Rat)

Alternate Names

glutamate receptor, metabotropic 5; GPRC1E; GPRC1EMGLUR5metabotropic glutamate receptor 5; GRM5; mGlu5; mGluR5